Figure 1 given below shows an ideal growth curve for microorganisms growing in a batch activated sludge system. During their life cycle, the microorganisms undergo a continuously changing cycle of growth and decline. In the proper environment the microorganisms convert the soluble and colloidal material present in the wastewater into new cells (activated sludge) and end products (CO 2 and water). The basic purpose of the activated sludge system is to establish and maintain a viable population of microorganisms by supplying food (BOD 5) and the proper environment. The basic reactions involve the breakdown of the organic matter (represented by 5 day Biochemical Oxygen Demand - BOD 5) and the formation of cell mass (activated sludge) and by-products (carbon dioxide and water). In practice, MLSS represents all suspended matter in the activated sludge system including inert matter, non-biological organic matter, as well as active microorganisms.
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